کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5788530 1414253 2017 21 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A CD44-biosensor for evaluating metastatic potential of breast cancer cells based on quartz crystal microbalance
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
A CD44-biosensor for evaluating metastatic potential of breast cancer cells based on quartz crystal microbalance
چکیده انگلیسی
A sensitive CD44-biosensor based on quartz crystal microbalance (QCM) was proposed for evaluating metastatic potential of breast cancer cells by using hyaluronan (HA) functionalized substrate film, polydopamine and polyethyleneimine composite film, for the purpose of capturing CD44-positive cancer cells through specific binding of HA to CD44. Two differently CD44-expressed breast cancer cell lines (MDA-MB-231 cells and MCF-7 cells) were put to use as targets for quantitative analysis as well as evaluation of metastatic potential of the cells. The limit of detection for MDA-MB-231 (M231) cells and MCF-7 cells were 300 and 1,000 cells mL−1, respectively. The expression level of CD44 on M231 cells exhibited two times higher than that of MCF-7 cells, indicating of a higher metastatic potential. Moreover, poly-L-lysine modified QCM sensor was applied to monitor the stiffness of breast cancer cells that can reflect metastatic potential of cells. The results revealed that the MCF-7 cells were stiffer than M231 cells, implying that the M231 cells possessed higher metastatic potential. The proposed protocol is simple and rapid to evaluate the metastatic potential of cancer cells, in addition to offering a promising diagnostic tool for metastatic cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science Bulletin - Volume 62, Issue 13, 15 July 2017, Pages 923-930
نویسندگان
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