کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5791660 1109616 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The protection of bovine skeletal myofibrils from proteolytic damage post mortem by small heat shock proteins
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
The protection of bovine skeletal myofibrils from proteolytic damage post mortem by small heat shock proteins
چکیده انگلیسی
This study aimed to determine how small heat shock proteins (sHSPs) protect myofibrillar proteins from μ-calpain degradation during ageing. Immunoprecipitation experiments with M. longissimus dorsi (LD) from Angus heifers (n = 14) examined the interaction between αβ-crystallin, desmin, titin, HSP20, HSP27 and μ-calpain. Results showed that αβ-crystallin associated with desmin, titin, HSP20, HSP27 and μ-calpain. Exogenous αβ-crystallin reduced desmin and titin degradations in myofibrillar extracts and attenuated μ-calpain activity. In a second experiment, bull LD (n = 94) were aged at − 1.5 °C for up to 28 days post mortem. μ-Calpain autolysed faster in high ultimate pH (pHu) meat (pHu ≥ 6.2) and this was concomitant with the more rapid degradation of titin and filamin in this pHu group. Desmin stability in intermediate pHu meat (pHu 5.8 to 6.19) may be due to the protection of myofibril-bound sHSPs combined with the competitive inhibition of μ-calpain by sHSPs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Meat Science - Volume 97, Issue 4, August 2014, Pages 548-557
نویسندگان
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