The azo dye Disperse Orange 1 induces DNA damage and cytotoxic effects but does not cause ecotoxic effects in Daphnia similis and Vibrio fischeri

► The dye Disperse Orange 1 induces mainly frameshift mutations in the DNA. ► This compound causes apoptosis and DNA breakage in the human cell HepG2. ► No toxic effects were detected for Daphnia similis and Vibrio fischeri.