Decolorization of different dyes by a newly isolated white-rot fungi strain Ganoderma sp.En3 and cloning and functional analysis of its laccase gene

► Ganoderma sp.En3 had a strong ability of decolorizing different dyes and dye effluents. ► The laccase gene lac-En3-1 and its corresponding full-length cDNA were cloned and characterized. ► lac-En3-1 gene was successfully expressed in the heterologous Pichia pastoris system. ► The recombinant laccase produced by the yeast transformant could decolorize different dyes and dye effluents.