کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5794872 1110501 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel bacterial phylotypes associated with the healthy feline oral cavity and feline chronic gingivostomatitis
ترجمه فارسی عنوان
فیتوتایپ های باکتریایی جدید در ارتباط با حفره های دهان و دندان سالم گربه و گینویواستوماتیت مزمن گربه
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی

Feline chronic gingivostomatitis (FCGS) is a painful inflammatory disease of the oral cavity. Treatment options for FCGS are very limited and little is known regarding its aetiology. The aim of this study was to investigate the presence of putative novel species in the oral cavity of cats with and without FCGS. Bacterial DNA was extracted from oral swabs and identified by 16S rRNA gene sequencing. The 16S rRNA genes of 54 clones representing distinct potentially novel species were sequenced (1202-1325 base pairs). Obtained sequences were compared to the BLAST database, aligned using the ClustalW2 alignment tool and a phylogenetic tree created. Twenty-two clones (18 from control and four from FCGS samples) had a similarity of less than 97% and were considered novel. The proportion of novel phylotypes in each group was 19.6% (control) and 2.3% (FCGS). In the derived phylogenetic tree, 15 novel phylotypes clustered together and branched away from known species and phyla. This suggests the presence of a group of novel, previously unidentified bacteria that are associated with the feline oral cavity in both health and disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Veterinary Science - Volume 94, Issue 3, June 2013, Pages 428-432
نویسندگان
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