Use of FTA technology for detection of Trichomonas gallinae

- In this study, we evaluated non-indicating FTA Elute micro cards as a potential sampling substrate for the molecular identification of Trichomonas gallinae isolates.
- Isolate concentrations of 10 or 100 trichomonads/40 μl, from two T. gallinae isolates were inoculated onto a FTA Elute cards and analyzed by conventional polymerase chain reaction (PCR) using trichomonad-specific primers at 48 h, 2 weeks, and 3 weeks post inoculation.
- Three PCR-positive samples were detected at 48 h from one isolate; however, all eluents from cards held for 2-3 weeks were PCR-negative. Our results suggest that FTA Elute cards have a low sensitivity for molecular identification of T. gallinae in low concentrations, such as those found in non-clinical birds; however, more research is needed to fully evaluate the efficacy of FTA Elute cards as a diagnostic tool for T. gallinae.

Trichomonas gallinae is the causative agent for avian trichomonosis, which can have important population implications for domestic turkeys, columbids, raptors, and various passeriformes. Continued population surveillance and genotype distribution is needed to elucidate transmission dynamics and prevalence of T. gallinae among free-ranging birds. However, obtaining live cultures for laboratory testing is logistically challenging, limiting the ability to perform surveillance and genotype investigations. In this study, we evaluated non-indicating FTA Elute cards as a potential sampling storage substrate for downstream use in molecular identification of two T. gallinae isolates. Isolate concentrations of 10 or 100 trichomonads/40 μl were inoculated onto a FTA Elute card in triplicate. At each time point (48 h, 2 weeks, and 3 weeks), DNA elution procedures were performed on the cards, and the eluents were analyzed by conventional polymerase chain reaction (PCR) using trichomonad-specific primers. Three PCR-positive samples were detected at 48 h from one isolate; however, all eluents from cards held for 2 and 3 weeks were PCR-negative. Our results suggest that use of FTA Elute cards for nucleic acid storage can lead to low PCR sensitivity of T. gallinae in low concentrations, such as those found in non-clinical birds; however, more research is needed to fully evaluate the efficacy of FTA Elute cards as a diagnostic tool for T. gallinae.