کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5802532 1555674 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transfection of live, tick derived sporozoites of the protozoan Apicomplexan parasite Theileria parva
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Transfection of live, tick derived sporozoites of the protozoan Apicomplexan parasite Theileria parva
چکیده انگلیسی


- We present the first transient transfection of the parasite.
- Plasmid DNA was integrated by nucleofection into fresh purified.
- Transfection will facilitate research of the unique host-parasite interactions.

Theileria parva is an important veterinary protozoan causing the tick-borne disease East Coast fever. Transfection of Theileria parasites will facilitate the investigation of many aspects of this apicomplexan infection and its unique host-parasite interaction. The pathogen has the extraordinary capacity of transforming B and T cells into clonally dividing cancerous cell lines in a reversible way. Sequence data of the entire T. parva genome are available and in vitro infected cell lines can easily be generated, thereby eliminating the use of animals in the evaluation of the evolution of the transfected sporozoites. Here we report, for the first time, on experiments towards successful transient transfection of T. parva sporozoites, making use of a new generation transfection device. Plasmid DNA containing the strong EF-1α promoter and an Azami Green reporter gene were integrated by nucleofection into freshly purified T. parva sporozoites. Expression of Azami Green was detected with a fluorescence microscope and confirmed by counter staining with a monoclonal directed against a sporozoite protein. Despite the fact that transfection efficiencies are still low, this is the first step towards a stable infection method of T. parva parasites. In the long run, transfected parasites might become an alternative way to induce immunity without clinical signs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 208, Issues 3–4, 15 March 2015, Pages 238-241
نویسندگان
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