کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5803044 1555680 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro and in vivo safety and efficacy studies of amphotericin B on Babesia gibsoni
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
In vitro and in vivo safety and efficacy studies of amphotericin B on Babesia gibsoni
چکیده انگلیسی


- Amphotericin B exhibits in vitro activity against B. gibsoni.
- Liposomal amphotericin B inhibited invasion of free parasites into erythrocytes.
- Amphotericin B induced mild hemolysis of canine erythrocytes in vitro.
- Amphotericin B has in vivo activity against B. gibsoni.
- Amphotericin B affects kidney function at a high dose.

Babesia gibsoni is a causative pathogen of canine babesiosis, which is commonly treated with anti-babesial drugs; however, the development of novel, more effective anti-babesial drugs is necessary because the currently used drugs cannot remove the parasites from dogs. Therefore we investigated the anti-babesial effect of amphotericin B (AmB), a membrane-active polyene macrolide antibiotic. The interaction of such compounds with sterols in bilayer cell membranes can lead to cell damage and ultimately cell lysis. AmB exhibits in vitro activity against B. gibsoni in normal canine erythrocytes within 12 h. We also studied liposomal AmB (L-AmB), a liposomal formulation of AmB that required a longer incubation period to reduce the number of parasites. However, L-AmB completely inhibited the invasion of free parasites into erythrocytes. These results indicated that free parasites failed to invade erythrocytes in the presence of L-AmB. Both AmB and L-AmB induced mild hemolysis of erythrocytes. Moreover, the methemoglobin level and the turbidity index of erythrocytes were significantly increased when erythrocytes were incubated with AmB, suggesting that AmB induced oxidative damage in erythrocytes. Finally, the anti-babesial activity of AmB in vivo was observed. When experimentally B. gibsoni-infected dogs were administered 0.5 and 1 mg/kg AmB by the intravenous route, the number of parasites decreased; however, recurrence of parasitemia was observed, indicating that AmB did not eliminate parasites completely. Blood urea nitrogen and creatinine of dogs were abnormally elevated after the administration of 1 mg/kg AmB. These results indicate that AmB has in vivo activity against B. gibsoni; however, it does not eliminate parasites from infected dogs and affects kidney function at a high dose.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 205, Issues 3–4, 15 October 2014, Pages 424-433
نویسندگان
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