کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5803307 1555689 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Eimeria tenella rhomboid 3 has a potential role in microneme protein cleavage
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Eimeria tenella rhomboid 3 has a potential role in microneme protein cleavage
چکیده انگلیسی

Invasion in several apicomplexan parasites, including Eimeria tenella, is accompanied by shedding of surface adhesins by intramembrane proteolysis mediated by rhomboid protease. We have previously identified E. tenella rhomboid 3 (EtROM3), but its precise role has not been elucidated. In this study, the interactions between EtROM3 and microneme (MIC) proteins were analyzed using the yeast two hybrid technique. The results showed that c-Myc-ROM3 fusion protein interacted with EtMIC4 protein in co-transformed AH109 yeasts, which was further confirmed by immunoprecipitation assay. Smaller EtMIC4 band from co-transformed cells suggested that EtROM3 was an active protease and involved in the cleavage of EtMIC4.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 201, Issues 1–2, 17 March 2014, Pages 146-149
نویسندگان
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