Evaluation of the use of pooled fecal samples for the diagnosis of protostrongylid infections in sheep

The Baermann-Wetzel method is the recommended test for the diagnosis of lungworm infections. The objective of this study was to evaluate the use of pooled fecal samples for the diagnosis of protostrongylid infections in sheep flocks and to investigate the sensitivity of the pooled Baermann-Wetzel technique in relation to individual analysis, which is time consuming and expensive. Fecal samples were taken from 10 sheep flocks positive for protostrongylids located in northwestern Spain. Ten pools composed of 6 individual fecal samples, chosen at random from the entire flock sampling, were performed for each flock at the same time as individual analyses. Protostrongylid larvae were detected in 56 pools, with a 56% flock sensitivity. Flock sensitivity was positively associated with the within-flock prevalence (P < 0.001), but not with the mean larvae output. A high sensitivity (78.3%) has been observed in flocks with medium or high prevalence, so pooled fecal samples can be used in those flocks that require an effective treatment regimen against these nematodes.