کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5805205 1555710 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Establishment and evaluation of an iELISA using the recombinant membrane protein LHD-Sj23 for the serodiagnosis of Schistosoma japonicum infection in cattle in China
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Establishment and evaluation of an iELISA using the recombinant membrane protein LHD-Sj23 for the serodiagnosis of Schistosoma japonicum infection in cattle in China
چکیده انگلیسی

Schistosomiasis is an important zoonosis and some livestock especially cattle play a crucial role in disease transmission in endemic areas. In order to establish an effective diagnostic method for detecting Schistosoma japonicum infection in cattle, the gene encoding large hydrophilic domain of Sj23 (LHD-Sj23) was cloned and expressed in Escherichia coli as a fusion protein with GST tag. The purified rLHD-Sj23-GST was used as an antigen in an indirect enzyme-linked immunosorbent assay (iELISA). The sera samples collected from cattle experimentally infected with S. japonicum from week 0 to week 54 post-infection were examined by rLHD-Sj23-GST iELISA. Furthermore, 484 clinical sera samples collected from cattle in schistosomiasis epidemic and free areas in China were tested by this method with a positive rate of 18.4% and 3.44%, respectively. The findings from this study indicated the established iELISA is a useful method for diagnosing S. japonicum infection in cattle and could be used in serological surveys to map out the prevalence of this disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 188, Issues 3–4, 10 September 2012, Pages 247-254
نویسندگان
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