ReviewModeling anxiety using adult zebrafish: A conceptual review

Zebrafish (Danio rerio) are rapidly emerging as a useful animal model in neurobehavioral research. Mounting evidence shows the suitability of zebrafish to model various aspects of anxiety-related states. Here, we evaluate established and novel approaches to uncover the molecular substrates, genetic pathways and neural circuits of anxiety using adult zebrafish. Experimental approaches to modeling anxiety in zebrafish include novelty-based paradigms, pharmacological and genetic manipulations, as well as innovative video-tracking, 3D-reconstructions, bioinformatics-based searchable databases and omics-based tools. Complementing traditional rodent models of anxiety, we provide a conceptual framework for the wider application of zebrafish and other aquatic models in anxiety research.This article is part of a Special Issue entitled 'Anxiety and Depression'.

► Zebrafish are useful for studying the underpinnings of neurobehavioral disorders. ► Current and emerging approaches used to uncover anxiety mechanisms are discussed. ► Pharmacological and genetic manipulations have validated zebrafish anxiety models. ► New and innovative bioinformatic approaches are emerging tools in anxiety research. ► Inter-species comparisons are key for uncovering pathogenic mechanisms.