کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5827992 | 1558946 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cannabinoid and lipid-mediated vasorelaxation in retinal microvasculature
ترجمه فارسی عنوان
واسنجی کانابینوئید و لیپیدها در میکروارگانیسم شبکیه
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
AM630MicrovasculatureGPR55AM251iberiotoxinIbTXSKCaCB2ACEAET-1CB1ETACBENOSLPIO-1602BKCa - BKC بهl-NAME - L-NAMEendothelin-1 - اندوتلین-1Retina - شبکیهNAgly - ناگلیNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازCannabinoid - کانابینوئیدLarge-conductance calcium-activated potassium channel - کانال پتاسیم فعال کلسیم با کارایی بالاSmall-conductance calcium-activated potassium channel - کانال پتاسیم فعال کلسیم کوچکCannabinoid receptor 1 - گیرنده کانابینوئید 1cannabinoid receptor 2 - گیرنده کانابینوئید 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
چکیده انگلیسی
The endocannabinoid system plays a role in regulation of vasoactivity in the peripheral vasculature; however, little is known about its role in regulation of the CNS microvasculature. This study investigated the pharmacology of cannabinoids and cannabimimetic lipids in the retinal microvasculature, a CNS vascular bed that is autoregulated. Vessel diameter (edge detector) and calcium transients (fura-2) were recorded from segments of retinal microvasculature isolated from adult, male Fischer 344 rats. Results showed that abnormal cannabidiol (Abn-CBD), an agonist at the putative endothelial cannabinoid receptor, CBe, inhibited endothelin 1 (ET-1) induced vasoconstriction in retinal arterioles. These actions of Abn-CBD were independent of CB1/CB2 receptors and were not mediated by agonists for GPR55 or affected by nitric oxide synthase (NOS) inhibition. However, the vasorelaxant effects of Abn-CBD were abolished when the endothelium was removed and were inhibited by the small Ca2+-sensitive K channel (SKCa) blocker, apamin. The effects of the endogenous endocannabinoid metabolite, N-arachidonyl glycine (NAGly), a putative agonist for GPR18, were virtually identical to those of Abn-CBD. GPR18 mRNA and protein were present in the retina, and immunohistochemistry demonstrated that GPR18 was localized to the endothelium of retinal vessels. These findings demonstrate that Abn-CBD and NAGly inhibit ET-1 induced vasoconstriction in retinal arterioles by an endothelium-dependent signaling mechanism that involves SKCa channels. The endothelial localization of GPR18 suggests that GPR18 could contribute to cannabinoid and lipid-mediated retinal vasoactivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 735, 15 July 2014, Pages 105-114
Journal: European Journal of Pharmacology - Volume 735, 15 July 2014, Pages 105-114
نویسندگان
Jessica MacIntyre, Alex Dong, Alex Straiker, Jiequan Zhu, Susan E. Howlett, Amina Bagher, Eileen Denovan-Wright, Dao-Yi Yu, Melanie E.M. Kelly,