Suppression of LPS-induced inflammatory responses by gossypol in RAW 264.7 cells and mouse models

► Gossypol reduced the production of TNF-α, IL-6 and IL-1β in LPS-stimulated RAW 264.7 macrophages. ► Gossypol modulated cytokine responses by blocking NF-қB and MAPKs activation. ► Gossypol attenuated TNF-α, IL-1β and IL-6 production in BALF of mice challenged with LPS. ► Gossypol decreased the number of total cells, neutrophils, and macrophages in BALF of mice treated with LPS. ► Gossypol attenuated the pulmonary histological changes and lung edema of mice treated with LPS.