Ad-KDRscFv:sTRAIL displays a synergistic antitumor effect without obvious cytotoxicity to normal tissues

► We investigate the antitumor activities and safety of Ad-KDRscFv: sTRAIL. ► We established three adenovirus particles: Ad-KDRscFv, Ad-sTRAIL, and Ad-KDRscFv:sTRAIL. ► Ad-KDRscFv:sTRAIL showed the strongest tumoricidal effect by inhibiting the tumor growth and angiogenesis. ► KDRscFv:sTRAIL had no obvious toxicity to normal cells and tissues in vitro and in vivo. ► KDRscFv:sTRAIL may be useful in tumor therapy.