کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5833627 | 1122628 | 2012 | 6 صفحه PDF | دانلود رایگان |

Cordycepin is reported to participate in multiple pharmacological activities including anti-tumor and anti-inflammation, and is involved in the regulation of NF-κB signaling pathway. However, the detailed molecular mechanism of cordycepin in suppression of NF-κB signaling pathway remains ambiguous. In this study, we first analyzed the effect of cordycepin on NF-κB activity in HEK-293T cells, and found that cordycepin resulted in a dose-dependent reduction in TNF-α-induced NF-κB activation. Although cordycepin did not block TNF-α-induced nuclear translocation of p65, high concentration of cordycepin reduced the DNA-binding and transcriptional activities of NF-κB. Moreover, cordycepin also inhibited IκBα phosphorylation so as to suppress the degradation of IκBα. Further investigation revealed that cordycepin suppressed IKKs-mediated NF-κB activation and inhibited the ubiquitination of IKKγ. In conclusion, cordycepin effectively inhibits NF-κB signaling through suppressing the activities of NF-κB, IκB and IKK. Thus, cordycepin may provide some potential therapeutic application in inflammation-associated disorders and cancer.
⺠Cordycepin resulted in a dose-dependent reduction in TNF-α-induced NF-κB activation. ⺠Cordycepin reduced p65 transcriptional activity at high concentration. ⺠Cordycepin inhibited IκBα phosphorylation. ⺠Cordycepin inhibited IKKs-mediated NF-κB activation. ⺠Cordycepin suppressed IKKγ ubiquitination in a dose-dependent manner.
Journal: International Immunopharmacology - Volume 14, Issue 4, December 2012, Pages 698-703