Preparation and functional identification of a monoclonal antibody against the recombinant soluble human NKp30 receptor

► The ectodomain of the human NKp30 receptor is recombinant expressed and purified. ► The activity of rhNKp30 is confirmed by flow cytometry and NK cytotoxicity assays. ► The anti-rhNKp30 monoclonal antibodies are obtained. ► The anti-rhNKp30 monoclonal antibodies can be used in western blotting, immunoprecipitation, ELISA, and flow cytometry assays.