Non-ionic surfactant vesicles mediated transcutaneous immunization against hepatitis B

► Topical immunization enables the use of antigens and adjuvant onto the skin to provide a novel approach of vaccination. ► Investigational study of niosomes to evoke systemic immune response against HBsAg by transcutaneous immunization. ► Topical immunization using niosomes with cell-binding B subunit of cholera toxin as an adjuvant is simple, stable and safe.