Covalent binding of nitroso-sulfonamides to glutathione S-transferase in guinea pigs with delayed type hypersensitivity

► Active metabolite of Sulfanilamide or Sulfamethoxazole is nitroso compound. ► Guinea pig glutathione S-transferase is a protein binded with the nitroso compound. ► Immunization of guinea pigs with the nitroso compound may give informations. ► Nitroso-glutathione S-transferase acts as a neoantigen in the body of guinea pigs.