کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5834241 1122667 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of TLR4 and TCR or BCR against baicalin-induced responses in T and B cells
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Role of TLR4 and TCR or BCR against baicalin-induced responses in T and B cells
چکیده انگلیسی

Baicalin (BA), a flavonoid compound isolated from Scutellaria baicalensis, has been shown to possess a number of pharmacological effects including antiviral, anti-inflammatory, antioxidant and immune regulation. Here, we examined its effects on human T and B cells proliferation by MTT assay and found that BA stimulated T and B cells proliferation, independently and cooperatively with Con-A (T cells) or LPS (B cells). Then, we analyzed the effects of BA treatment on the mRNA expression of Toll-like receptors (TLRs), IL-2, IFN-γ and IL-12 in T and B cells by real-time RT-PCR and attempted to observe whether blocking TLR4 had influence on mRNA expression. We found that BA treatment significantly up-regulated TLR3, 7, 8 and 9 mRNA expressions in T and B cells, IL-2 and IFN-γ in T cells and IL-12 in B cells. The increased mRNA expressions were suppressed after blocking TLR4. We further analyzed the effects of BA treatment on TCR vβ and CD79 mRNA expression levels in T and B cells and explored whether blocking TCR (αβ) or BCR mIgM F(ab′)2 had an influence on mRNA expression. We found that BA treatment significantly improved TCR vβ and CD79 mRNA expression in T and B cells, respectively, and the improvements were all inhibited after blocking TCR (αβ) or BCR mIgM F(ab′)2. Our results suggested that BA participates in innate and adaptive immune regulation by up-regulating the mRNA expression of TLRs (3, 7, 8 and 9), IL-2, IFN-γ and IL-12 in T and B cells, which is mediated by TLR4, and by improving the mRNA expression of TCR vβ and CD79, which is mediated by TCR (αβ) and BCR mIgM, respectively. Therefore, TLR4, TCR (αβ) and BCR mIgM are all the immune receptors for BA on T and B cells.

► BA stimulates T and B cells proliferation independently and cooperatively with Con-A or LPS. ► BA up-regulates TLRs(3,7,8and9) mRNA expression in T and B cells mediated by TLR4. ► BA up-regulates IL-2, IFN-γ and IL-12 mRNA expression in T and B cells mediated by TLR4. ► BA improves TCR vβ and CD79 mRNA expression mediated by TCR (αβ) and BCR mIgM respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 11, Issue 12, December 2011, Pages 2176-2180
نویسندگان
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