Application of response surface methodology for optimization of lead biosorption in an aqueous solution by Aspergillus niger

Response surface methodology was applied to optimize the removal of lead ion by Aspergillus niger in an aqueous solution. Experiments were conducted based on a rotatable central composite design (CCD) and analyzed using response surface methodology (RSM). The biosorption process was investigated as a function of three independent factors viz. initial solution pH (2.8-7.2), initial lead concentration (8-30 mg/l) and biomass dosage (1.6-6 g/l). The optimum conditions for the lead biosorption were found to be 3.44, 19.28 mg/l and 3.74 g/l, respectively, for initial solution pH, initial lead ion concentration and biomass dosage. Lead biosorption capacity on dead A. niger fungal biomass was enhanced by pretreatment using NaOH. Under these conditions, maximum biosorption capacity of the biomass for removal of lead ions was obtained to 96.21%. The desirability function was used to evaluate all the factors and response in the biosorption experiments in order to find an optimum point where the desired conditions could be obtained. The A. niger particles with clean surface and high porosity may have application as biosorbent for heavy metal removal from wastewater effluents.