کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5838195 | 1123977 | 2012 | 4 صفحه PDF | دانلود رایگان |
Ethnopharmacological relevanceRhizome of Zingiber montanum has been extensively used as a folk medicine to ameliorate peptic ulcer at northern part of Bangladesh.Aim of the studyTo identify the antiulcer principle of the MeOH extract of the rhizome of Zingiber montanum by an ex vivo bioassay guided chromatographic separation and purification, and structure elucidation of the purified compound by spectroscopic methods.Materials and methodsDried powder of Zingiber montanum rhizomes was extracted with MeOH. The antiulcer activity of the crude extract and its chromatographic fractions were evaluated by the inhibition of 1Â N HCl induced gastric lesions in Swiss albino mice. The pure compound was purified from the active fraction by crystallization with hexanes. Structure of the pure compound was elucidated by spectroscopic methods. The antiulcer activity of the pure compound was evaluated by the inhibition of 1Â N HCl, 95% ethanol and indomethacin induced gastric lesions in mice.ResultsThe MeOH extract of Zingiber montanum showed 61.97% and 83.10% inhibition of the 1Â N HCl induced gastric lesions at doses of 200Â mg/kg and 400Â mg/kg, respectively, in mice. Chromatographic separation on silica gel of the extract was yielded seven fractions and the fraction 2 was found to have most potent antiulcer activity in mice. This fraction showed 77.46% inhibition of the 1Â N HCl induced gastric lesions at a dose of 40Â mg/kg in mice. Crystallization of the fraction yielded 1 (zerumbone, 180Â mg). It showed statistically 45.77% and 92.25% inhibition of 1Â N HCl induced gastric lesions in mice at doses of 20Â mg/kg and 40Â mg/kg, respectively. It also showed 29.07% and 45.35% inhibition of 95% ethanol induced gastric mucosal damage, and 64.76% and 72.38% inhibition of indomethacin induced gastric lesions in mice at doses of 20Â mg/kg and 40Â mg/kg, respectively.ConclusionZerumbone (1) showed potent cytoprotective effect against necrotizing agent (HCl) and non-steroidal anti-inflammatory drug (indomethacin) induced gastric ulceration. It also exhibited moderate cytoprotective effect against noxious agent (EtOH) induced gastric lesions. It can be considered as a promising new antiulcer natural drug lead.
Antiulcer activity guided fractionations and purification of the rhizomes of Zingiber montanum yielded zerumbone (1) as active principle of the medicinal plant. It inhibited HCl, ethanol and indomethacin induced gastric lesions in mice dose dependently.226
Journal: Journal of Ethnopharmacology - Volume 141, Issue 1, 7 May 2012, Pages 57-60