Mechanisms of anti-inflammatory effect of an active ingredients group from Jinxuan Zhike Xunxi San

Aim of the studyJZXS has been used for inflamed hemorrhoids as a chinese prescription for several decades. The present study was designed to investigate the anti-inflammatory activities, as well as the mechanisms, of an active ingredients group (AIG) obtained from Jinxuan Zhike Xunxi San (JZXS).Materials and methodsThe anti-inflammatory activities and mechanisms of AIG were evaluated by xylene-induced ear edema experiments in normal mice and mice without adrenals, leukocyte migration experiments, and carrageenin-induced peritonitis experiments, taking JZXS as the positive control.ResultsAIG and JZXS prevented xylene-induced ear edema in normal mice but showed no effects when adrenals were removed. Additionally, AIG and JZXS inhibited leukocyte migration, reduced prostaglandin E2 (PGE2) level in inflammatory exudates and nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 and 8 (IL-6 and IL-8) levels in serum.ConclusionsAIG and JZXS showed significant anti-inflammatory activities depending on pituitary-adrenal axis, thereby inhibiting leukocyte migration and reducing cytokines and mediators.

Anti-inflammatory activities as well as the mechanisms of an active ingredients group (AIG) from Jinxuan Zhike Xunxi San (JZXS) were evaluated taking JZXS as the positive control.200