کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5846152 1561173 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protective effects of myricitrin against osteoporosis via reducing reactive oxygen species and bone-resorbing cytokines
ترجمه فارسی عنوان
اثرات محافظتی میریریکین در برابر پوکی استخوان از طریق کاهش میزان اکسیژن واکنش پذیر و سیتوکین های استخوانی
کلمات کلیدی
مریریکین، گونه های اکسیژن واکنش پذیر، سیتوکین استخوانی، پوکی استخوان، موس تخمدان سلول استروما مغز استخوان انسان،
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


- Myricitrin protects MC3T3-E1 cells and hBMSCs from oxidative stress.
- It is accompanied by a decrease in oxidative stress and bone-resorbing cytokines.
- Myricitrin decreases serum reactive oxygen species to some degree.
- Myricitrin partly reverses ovariectomy effects in vivo.
- Myricitrin may represent a beneficial anti-osteoporosis treatment method.

Oxidative stress is a crucial pathogenic factor in the development of osteoporosis. Myricitrin, isolated from Myrica cerifera, is a potent antioxidant. We hypothesized that myricitrin possessed protective effects against osteoporosis by partially reducing reactive oxygen species (ROS) and bone-resorbing cytokines in osteoblastic MC3T3-E1 cells and human bone marrow stromal cells (hBMSCs). We investigated myricitrin on osteogenic differentiation under oxidative stress. Hydrogen peroxide (H2O2) was used to establish an oxidative cell injury model. Our results revealed that myricitrin significantly improved some osteogenic markers in these cells. Myricitrin decreased lipid production and reduced peroxisome proliferator-activated receptor gamma-2 (PPARγ2) expression in hBMSCs. Moreover, myricitrin reduced the expression of receptor activator of nuclear factor kappa-B ligand (RANKL) and IL-6 and partially suppressed ROS production. In vivo, we established a murine ovariectomized (OVX) osteoporosis model. Our results demonstrated that myricitrin supplementation reduced serum malondialdehyde (MDA) activity and increased reduced glutathione (GSH) activity. Importantly, it ameliorated the micro-architecture of trabecular bones in the 4th lumbar vertebrae (L4) and distal femur. Taken together, these results indicated that the protective effects of myricitrin against osteoporosis are linked to a reduction in ROS and bone-resorbing cytokines, suggesting that myricitrin may be useful in bone metabolism diseases, particularly osteoporosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 280, Issue 3, 1 November 2014, Pages 550-560
نویسندگان
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