کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5848715 1561704 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Individual and combined effects of deoxynivalenol and α-zearalenol on cell proliferation and steroidogenesis of granulosa cells in cattle
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Individual and combined effects of deoxynivalenol and α-zearalenol on cell proliferation and steroidogenesis of granulosa cells in cattle
چکیده انگلیسی


- Deoxynivalenol and zearalenone are mycotoxins produced by Fusarium species.
- Granulosa cells were used to test the effects of deoxynivalenol and α-zearalenol.
- Deoxynivalenol and α-zearalenol were tested alone and in combination.
- Deoxynivalenol and α-zearalenol had effects on in vitro granulosa cells function.
- Further studies are needed to better understand their effects on bovine species.

This study was conducted to evaluate the impact of deoxynivalenol (DON) and zearalenone (ZEA) metabolite, α-zearalenol (α-Zol), on cell proliferation and steroidogenesis of bovine large (LG) follicle granulosa cells (GC). LGGC were obtained from bovine ovarian follicles (8-22 mm) and were cultured for 2 days in medium containing 10% fetal bovine serum followed by 1 or 2 days in serum-free medium without (control) or with treatments. Three different experiments were performed using different dosages of DON and α-Zol and in different combinations and a fourth experiment evaluated estradiol effects on granulosa cell proliferation. DON inhibited progesterone (P4) and estradiol (E2) production at high dose. α-Zol alone and in combination with DON increased cell growth. Estradiol inhibited cell growth indicating α-Zol is not acting as an estrogen agonist. This study demonstrates that α-Zol and DON can impact in vitro GC function, however further studies will be required to better understand the mechanism of action and reproductive effects of Fusarium mycotoxins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 40, Issue 3, November 2015, Pages 722-728
نویسندگان
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