کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5848901 1130683 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Toxic effect of cooking oil fumes in primary fetal pulmonary type II-like epithelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Toxic effect of cooking oil fumes in primary fetal pulmonary type II-like epithelial cells
چکیده انگلیسی


- We investigated Cytotoxic and genotoxic effects of COFs on AEC II cells.
- Oxidative damage was shown when exposed to different doses of COFs.
- DNA adducts and DNA damage were shown after exposure to COFs.
- We also detected cell cycle disturbance in the present study.

Epidemiological studies indicated that there is an increased risk of respiratory tract cancer among cooks and bakers. The cooking oil fumes are believed to conduct this risk, and many studies have focused on evaluating the mutagenicity and finding the mutagenic components in oil fumes. COFs contains two major classes of compounds. One class consists of polycyclic aromatic hydrocarbons (PAHs), such as benzo[a]pyrene, benzo[b]fluoranthene, fluoranthene, and benzo[g,h,i]perylene. BaP is a known immunosuppressant. It can also alter cell cycle progression, induce inflammation, and impair DNA repair and apoptotic processes leading to aberrant cellular functioning. This study investigates the effect of toxicity of cooking oil fumes (COFs) in primary ICR mice' fetal lung type II-like epithelium cells (AEC II). The cells were cultured in different concentrations (0, 12.5, 25, 50, 100, and 200 μg/ml) of COFs for different time periods. The results showed that cell viability decreased in a dose- and time- dependent manner, which is accompanied by increased malondialdehyde (MDA) level and decreased superoxide dismutase (SOD) and glutathione (GSH) activities. Moreover, comet assay suggested DNA damage, as well as increased production of DNA adducts induced by PAHs. The present study also shows that COFs may disturb cell cycles even at a very low dose. In summary, the present study indicates that COFs may lead to toxicity in AEC II cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 36, Issue 2, September 2013, Pages 320-331
نویسندگان
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