کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5849427 | 1561756 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Safety evaluation of nuclease P1 from Penicillium citrinum
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کلمات کلیدی
inosine-5′-monophosphateGLPTOSMHLWIMPCBS9-AAOECDCMPUMPPenicillium citrinumGMP2-aminoanthraceneAF-2NaN3AMP2-(2-furyl)-3-(5-nitro-2-furyl)acrylamideEDTA-2KMinistry of Health, Labour and Welfareguanosine-5′-monophosphate2-AA - 2 AAA/G - A / GDNA - DNA یا اسید دزوکسی ریبونوکلئیکadenosine-5′-monophosphate - آدنوزین 5'-مونوفسفرهdeoxyribonucleic acid - اسید deoxyribonucleicribonucleic acid - اسید ریبونوکلئیکRNA - اسید ریبونوکلئیکSafety - ایمنیGood Laboratory Practice - تمرین خوب آزمایشگاهیFood - خوراک یا غذاsodium azide - سدیم آیزیدSubchronic toxicity - سمیت زیر سونوگرافیGenotoxicity - سمیت ژنتیکیmitotic index - شاخص متیوتیکNuclease P1 - نیکلاز P1body weight - وزن بدن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Safety evaluation of nuclease P1 from Penicillium citrinum Safety evaluation of nuclease P1 from Penicillium citrinum](/preview/png/5849427.png)
چکیده انگلیسی
Nuclease P1 has been widely used in the food industry to enhance or create flavor. One commercial source of this enzyme is Penicillium citrinum, an anamorphic mesophilic fungus with a long history of safe use in Europe and Asia as a fermentation organism used in the production of ribonucleases. Given the intended use in food for human consumption, and noting its potential presence at trace levels in finished products, a series of safety studies including an in vitro Ames and chromosome aberration assay, an in vivo rat erythrocyte micronucleus assay and a 90-day oral toxicity study in rats were conducted. No mutagenic activity was observed in the Ames assay. Equivocal activity in the chromosome aberration assay was not replicated in the micronucleus assay at doses of up to 1007 mg total organic solids (TOS)/kg body weight (bw)/day. Following oral administration of nuclease P1 at dosages of 10.1, 101 or 1007 mg TOS/kg bw/day to Sprague-Dawley rats, no adverse effects on any study parameter were observed. The no-observed-adverse-effect level was considered to be 1007 mg TOS/kg bw/day. The results of the genotoxicity studies and subchronic rat study support the safe use in food production of nuclease P1 produced from P. citrinum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 88, February 2016, Pages 21-31
Journal: Food and Chemical Toxicology - Volume 88, February 2016, Pages 21-31
نویسندگان
Nobuo Okado, Kazushige Hasegawa, Fukutaro Mizuhashi, Barry S. Lynch, Trung D. Vo, Ashley S. Roberts,