کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5849668 1561757 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Different concentrations of kaempferol distinctly modulate murine embryonic stem cell function
ترجمه فارسی عنوان
غلظت های مختلف کپسفول به طور قابل توجهی عملکرد سلول های بنیادی جنینی مغز را مدول می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


- Kaempferol presents toxic effects for mESC at elevated concentrations.
- Toxic effects on mESC are partially due to apoptosis induction by kaempferol.
- At lower concentrations, kaempferol positively affect Nanog expression in mESC.
- mESC ineffective differentiate with intermediate kaempferol concentrations.

Kaempferol (3,4′,5,7-tetrahydroxyflavone) is a natural flavonoid with several beneficial and protective effects. It has been demonstrated that kaempferol has anticancer properties, particularly due to its effects on proliferation, apoptosis and the cell cycle. However, possible effects on pluripotent embryonic stem cell function have not yet been addressed. Embryonic stem cells have the ability to self-renew and to differentiate into all three germ layers with potential applications in regenerative medicine and in vitro toxicology. We show that exposure of murine embryonic stem cells (mESC) to high concentrations of kaempferol (200 μM) leads to decreased cell numbers, although the resulting smaller cell colonies remain pluripotent. However, lower concentrations of this compound (20 μM) increase the expression of pluripotency markers in mESCs. Mitochondrial membrane potential and mitochondrial mass are not affected, but a dose-dependent increase in apoptosis takes place. Moreover, mESC differentiation is impaired by kaempferol, which was not related to apoptosis induction. Our results show that low concentrations of kaempferol can be beneficial for pluripotency, but inhibit proper differentiation of mESCs. Additionally, high concentrations induce apoptosis and increase mitochondrial reactive oxygen species (ROS).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 87, January 2016, Pages 148-156
نویسندگان
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