کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5850227 | 1561768 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The furofuran-ring selectivity, hydrogen peroxide-production and low Km value are the three elements for highly effective detoxification of aflatoxin oxidase
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کلمات کلیدی
ITC3,4-Dihydro-2H-pyranN-hydroxysuccinimideDHP8-MOPAFONHSHRPSWCNTDPVEDCPBSAFB1 - AFB 1Aflatoxin B1 - آفلاتوکسین B1Sterigmatocystin - استریگماتوسیستینElectrochemistry - الکتروشیمیOxidase - اکسیدازBenzofuran - بنزوفورانFuran - فورانphosphate buffer solution - محلول بافر فسفاتSingle-walled carbon nanotube - نانولوله کربنی تک دیوارهDifferential pulse voltammetry - ولتامتری پالس تفاضلیCyclic voltammetry - ولتامتری چرخهایHorseradish peroxidase - پراکسیداز هوررادیشIsothermal titration calorimetry - کالری سنجی تیتاسیون ایزوترمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
AFO (aflatoxin oxidase), an enzyme from Armillariella tabescens previously named aflatoxin detoxifizyme, exhibits oxidative detoxification activity toward aflatoxin B1 and sterigmatocystin. Bioinformatics reveals that AFO is a newly discovered oxidase because AFO does not share any significant similarities with any known oxidase. It is critically important to understand how AFO acts on aflatoxin B1. In this study, in addition to aflatoxin B1 (AFB1) and sterigmatocystin (ST), five other chemicals that have furan or pyran structures were investigated. The results indicated that in addition to AFB1 and ST, AFO is also able to act on versicolorin A, 3,4-dihydro-2H-pyran and furan. These results suggested that 8,9-unsaturated carboncarbon bond of aflatoxin B1 is the potential reactive site for AFO. Further findings indicated that the action of AFO is oxygen-dependent and hydrogen peroxide-producing. The simultaneously produced-hydrogen peroxide possibly plays the essential role in detoxification of AFO. In addition, the extremely low Km value of 0.33âµmol/l for AFO-AFB1 and 0.11âµmol/l for AFO-ST signifies that AFO is highly selective for AFB1 as well as ST.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 76, February 2015, Pages 125-131
Journal: Food and Chemical Toxicology - Volume 76, February 2015, Pages 125-131
نویسندگان
Yuan-Zhen Wu, Fu-Pu Lu, Hai-Lan Jiang, Cui-Ping Tan, Dong-Sheng Yao, Chun-Fang Xie, Da-Ling Liu,