کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5850378 | 1561782 | 2013 | 6 صفحه PDF | دانلود رایگان |

- Tanshinone I inhibits peroxynitrite-induced DNA damage.
- Tanshinone I protects against peroxynitrite-mediated cytotoxicity.
- Tanshinone I scavenges hydroxyl radicals from peroxynitrite.
Tanshinone I, a naturally occurring diterpene from Danshen, has been shown to possess hepatocyte protective, anticancer, and memory enhancing properties. However, there are few stringent pharmacological tests for neuroprotection of tanshinone I thus far. Since peroxynitrite is involved in the pathogenesis of neurodegenerative disorders, this study was undertaken to investigate whether the neuroprotective effect of tanshinone I is associated with inhibition of peroxynitrite-caused DNA damage, a critical event leading to peroxynitrite-induced cytotoxicity. Our results show that tanshinone I can significantly inhibit peroxynitrite-induced DNA damage both in ÏX-174 plasmid DNA and rat primary astrocytes. EPR spectroscopy indicates that tanshinone I potently diminished the DMPO-hydroxyl radical adduct signal from peroxynitrite. Taken together, these results demonstrate for the first time that tanshinone I can protect against peroxynitrite-induced DNA damage, hydroxyl radical formation and cytotoxicity, which might have implications for tanshinone I-mediated neuroprotection.
Journal: Food and Chemical Toxicology - Volume 62, December 2013, Pages 407-412