کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5850957 | 1561780 | 2014 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Deoxynivalenol induced oxidative stress and genotoxicity in human peripheral blood lymphocytes
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کلمات کلیدی
PhytohemagglutininCytochalasin BMitomycin CHeme oxygenase-1l-glutathione reducedOECDBER8-OHdGDCFH-DADeoxynivalenolGSSGRT-PCRGSHHO-1PHASCEMMC5-Methylcytosine - 5-متیل سیتوزین8-hydroxydeoxyguanosine - 8 هیدروکسی دیواکسی گوآزینLC/MS - LC / MSROS - ROSOxidative damage - آسیب اکسیداتیوSister chromatid exchange - تبادل کروماتید خواهرDNA repair - ترمیم DNAbase excision repair - تعمیر پایه پایهDON - دونOrganization for Economic Cooperation and Development - سازمان همکاری های اقتصادی و توسعهGenotoxicity - سمیت ژنتیکیcyt b - سیت بHuman lymphocytes - لنفوسیت های انسانیmalonaldehyde - مالونالدئیدHemoglobin - هموگلوبینreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسLiquid chromatography/mass spectrometry - کروماتوگرافی مایع / طیف سنج جرمیhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کاراReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Deoxynivalenol induced oxidative stress and genotoxicity in human peripheral blood lymphocytes Deoxynivalenol induced oxidative stress and genotoxicity in human peripheral blood lymphocytes](/preview/png/5850957.png)
چکیده انگلیسی
Deoxynivalenol (DON) is one of the most common mycotoxins. The aim of this study consists in using diverse cellular and molecular assays to evaluate cytotoxicity, genotoxicity as well as oxidative damage and to investigate their mechanisms in human peripheral blood lymphocytes. The human lymphocytes were cultured in eight different doses of DON (0, 6.25, 12.5, 25, 50, 100, 250 and 500Â ng/mL) during 6, 12 and 24Â h. DON was able to decrease cell viability and cause damage to the membrane, the chromosomes or the DNA at all times of culture. It was also able to induce lipid peroxidation and raise the levels of 8-OHdG and ROS in 6, 12 and 24Â h. The results of the RT-PCR and the Western Blot indicated that DON is able to enhance mRNA or protein expressions of DNA repair genes and HO-1 in 6Â h and to inhibit these expressions in 24Â h. DON potentially triggers genotoxicity in human lymphocytes. This mechanism is probably related to depletion of antioxidase and oxidative damage to the DNA that reduced expression of HO-1, thereby inhibiting the ability of DNA repair.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 64, February 2014, Pages 383-396
Journal: Food and Chemical Toxicology - Volume 64, February 2014, Pages 383-396
نویسندگان
Wei Yang, Miao Yu, Juan Fu, Wei Bao, Di Wang, Liping Hao, Ping Yao, Andreas K. Nüssler, Hong Yan, Liegang Liu,