کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5854096 | 1130874 | 2011 | 4 صفحه PDF | دانلود رایگان |
Phenobarbital (PB) is an efficacious and well-studied hepatic tumor promoting agent. Nuclear factor-κB (NF-κB) is a transcription factor activated by reactive oxygen and is involved in cell proliferation and apoptosis. We previously found that PB activates NF-κB and that dietary vitamin E is effective in decreasing PB-induced NF-κB DNA binding. We therefore hypothesized that dietary vitamin E influences PB-induced changes in cell proliferation and apoptosis through its action on NF-κB. NF-κB1 deficient mice (p50â/â) and wild-type B6129 mice were fed a purified diet containing 10 or 250 ppm vitamin E (α-tocopherol acetate) for 28 days. At that time, half of the wild-type and half of the p50â/â mice were placed on the same diet with 0.05% PB for 10 days. Compared to wild-type mice, the p50â/â mice had higher levels of cell proliferation and apoptosis. Cell proliferation was significantly increased by PB, but vitamin E did not affect hepatic cell proliferation. Apoptosis was not changed in mice fed PB, and there was no significant difference in apoptosis between control and high vitamin E treated mice. Thus, vitamin E does not appear to influence cell growth parameters in either wild-type or p50â/â mice.
⺠Vitamin E did not significantly affect hepatocyte proliferation or apoptosis. ⺠Phenobarbital increased hepatocyte cell proliferation but not apoptosis. ⺠NF-κB p50 subunit deletion increased hepatocyte proliferation and apoptosis.
Journal: Food and Chemical Toxicology - Volume 49, Issue 10, October 2011, Pages 2706-2709