کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5858996 | 1562317 | 2016 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Systems toxicology-based assessment of the candidate modified risk tobacco product THS2.2 for the adhesion of monocytic cells to human coronary arterial endothelial cells
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کلمات کلیدی
E-selectinMM6Tobacco heating system 2.2MRTPTHS2.2HCAECsSELEVCAM-1TACEIPNFDRTNFαNPAICAM-1Atherosclerosis - آترواسکلروز(تصلب شریان)tumor necrosis factor-α-converting enzyme - آنزیم تبدیل کننده α-نکروز تومورtumor necrosis factor-alpha - تومور نکروز عامل آلفاCigarette smoke - دود سیگارhuman coronary artery endothelial cells - سلول اندوتلیال عروق کرونر انسانیEndothelial cell - سلول های اندوتلیالCigarette - سیگارInflammatory processes - فرآیندهای التهابیModified risk tobacco product - محصول تنباکو اصلاح شدهIntercellular adhesion molecule 1 - مولکول چسبندگی بین سلولی 1Vascular cell adhesion molecule 1 - مولکول چسبندگی سلولی عروقی 1Mono Mac 6 - مونو مک 6Monocyte - مونوسیتfalse discovery rate - میزان کشف کاذبAdhesion - چسبندگی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Systems toxicology-based assessment of the candidate modified risk tobacco product THS2.2 for the adhesion of monocytic cells to human coronary arterial endothelial cells Systems toxicology-based assessment of the candidate modified risk tobacco product THS2.2 for the adhesion of monocytic cells to human coronary arterial endothelial cells](/preview/png/5858996.png)
چکیده انگلیسی
Alterations of endothelial adhesive properties by cigarette smoke (CS) can progressively favor the development of atherosclerosis which may cause cardiovascular disorders. Modified risk tobacco products (MRTPs) are tobacco products developed to reduce smoking-related risks. A systems biology/toxicology approach combined with a functional in vitro adhesion assay was used to assess the impact of a candidate heat-not-burn technology-based MRTP, Tobacco Heating System (THS) 2.2, on the adhesion of monocytic cells to human coronary arterial endothelial cells (HCAECs) compared with a reference cigarette (3R4F). HCAECs were treated for 4Â h with conditioned media of human monocytic Mono Mac 6 (MM6) cells preincubated with low or high concentrations of aqueous extracts from THS2.2 aerosol or 3R4F smoke for 2Â h (indirect treatment), unconditioned media (direct treatment), or fresh aqueous aerosol/smoke extracts (fresh direct treatment). Functional and molecular investigations revealed that aqueous 3R4F smoke extract promoted the adhesion of MM6 cells to HCAECs via distinct direct and indirect concentration-dependent mechanisms. Using the same approach, we identified significantly reduced effects of aqueous THS2.2 aerosol extract on MM6 cell-HCAEC adhesion, and reduced molecular changes in endothelial and monocytic cells. Ten- and 20-fold increased concentrations of aqueous THS2.2 aerosol extract were necessary to elicit similar effects to those measured with 3R4F in both fresh direct and indirect exposure modalities, respectively. Our systems toxicology study demonstrated reduced effects of an aqueous aerosol extract from the candidate MRTP, THS2.2, using the adhesion of monocytic cells to human coronary endothelial cells as a surrogate pathophysiologically relevant event in atherogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 339, 2 January 2016, Pages 73-86
Journal: Toxicology - Volume 339, 2 January 2016, Pages 73-86
نویسندگان
Carine Poussin, Alexandra Laurent, Manuel C. Peitsch, Julia Hoeng, Hector De Leon,