کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5888466 1568022 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood
ترجمه فارسی عنوان
اثر دمای ذخیره سازی در یک محیط کامبوج روی ترکیب اسید چرب در کل خون
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
چکیده انگلیسی

Fatty acid analysis requires standardized collection and storage of samples, which can be a challenge under field conditions. This study describes the effect of storage temperature on fatty acid composition in two sets of whole blood samples collected from 66 children in a rural area in Cambodia. The samples were stored with butylated hydroxytoluene at −20 °C and −80 °C and the latter required extra transfers due to storage facility limitation. Fatty acid composition was analyzed by high-throughput gas-chromatography and evaluated by paired t-tests and Bland-Altman plots. Total amounts of fat in −20 °C and −80 °C samples did not differ, but there was relatively more highly unsaturated fatty acids (15.8±2.7 vs. 14.4±2.5%, p<0.001) and a lower n-6/n-3 ratio (6.4±1.4 vs. 6.9±1.4, p<0.001) in the −20 °C samples. Our results indicate that the importance of storage temperature should be evaluated in the context of storage facility availability and risk of temperature fluctuations during transport.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - Volume 96, May 2015, Pages 57-61
نویسندگان
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