کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5888466 | 1568022 | 2015 | 5 صفحه PDF | دانلود رایگان |
Fatty acid analysis requires standardized collection and storage of samples, which can be a challenge under field conditions. This study describes the effect of storage temperature on fatty acid composition in two sets of whole blood samples collected from 66 children in a rural area in Cambodia. The samples were stored with butylated hydroxytoluene at â20 °C and â80 °C and the latter required extra transfers due to storage facility limitation. Fatty acid composition was analyzed by high-throughput gas-chromatography and evaluated by paired t-tests and Bland-Altman plots. Total amounts of fat in â20 °C and â80 °C samples did not differ, but there was relatively more highly unsaturated fatty acids (15.8±2.7 vs. 14.4±2.5%, p<0.001) and a lower n-6/n-3 ratio (6.4±1.4 vs. 6.9±1.4, p<0.001) in the â20 °C samples. Our results indicate that the importance of storage temperature should be evaluated in the context of storage facility availability and risk of temperature fluctuations during transport.
Journal: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - Volume 96, May 2015, Pages 57-61