کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5889226 1568138 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lineage tracking of mesenchymal and endothelial progenitors in BMP-induced bone formation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Lineage tracking of mesenchymal and endothelial progenitors in BMP-induced bone formation
چکیده انگلیسی


- Tie2-lineage cells were tracked in BMP-2 induced bone using fluorescent report mice.
- Tie2-lineage cells contributed to vessels and osteoclasts but not osteoblasts.
- αSMA-lineage (mesenchymal) cells contributed to osteoblasts.
- Conditional deletion of Osx in Tie2-lineage cells did not affect BMP-2 induced bone.

To better understand the relative contributions of mesenchymal and endothelial progenitor cells to rhBMP-2 induced bone formation, we examined the distribution of lineage-labeled cells in Tie2-Cre:Ai9 and αSMA-creERT2:Col2.3-GFP:Ai9 reporter mice. Established orthopedic models of ectopic bone formation in the hind limb and spine fusion were employed. Tie2-lineage cells were found extensively in the ectopic bone and spine fusion masses, but co-staining was only seen with tartrate-resistant acid phosphatase (TRAP) activity (osteoclasts) and CD31 immunohistochemistry (vascular endothelial cells), and not alkaline phosphatase (AP) activity (osteoblasts). To further confirm the lack of a functional contribution of Tie2-lineage cells to BMP-induced bone, we developed conditional knockout mice where Tie2-lineage cells are rendered null for key bone transcription factor osterix (Tie2-cre:Osxfx/fx mice). Conditional knockout mice showed no difference in BMP-induced bone formation compared to littermate controls. Pulse labeling of mesenchymal cells with Tamoxifen in mice undergoing spine fusion revealed that αSMA-lineage cells contributed to the osteoblastic lineage (Col2.3-GFP), but not to endothelial cells or osteoclast populations. These data indicate that the αSMA + and Tie2 + progenitor lineages make distinct cellular contributions to bone formation, angiogenesis, and resorption/remodeling.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 81, December 2015, Pages 53-59
نویسندگان
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