کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5890111 | 1568157 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Notch1 and Notch2 expression in osteoblast precursors regulates femoral microarchitecture
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
μCTRANKLNotch1NOTCH2FABPSMIosterixOsteoblasts - استئوبلاست هاCortical bone - استخوان کورتیکHES - او هستOsx - اکسHairy enhancer of split - تقویت کننده مودار تقسیمMicrocomputed tomography - توموگرافی میکروکوموتیوstandard error of the mean - خطای استاندارد میانگینRank - رتبهStructure model index - شاخص ساختار مدلRANK ligand - لیگاند RANKSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیHey - هیFatty acid-binding protein - پروتئین اتصال دهنده اسید چربkilobase - کیلوبایز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Notch1 and Notch2 expression in osteoblast precursors regulates femoral microarchitecture Notch1 and Notch2 expression in osteoblast precursors regulates femoral microarchitecture](/preview/png/5890111.png)
چکیده انگلیسی
Notch receptors regulate cell differentiation and function. Notch1 and Notch2 inactivation in osteoblasts and osteocytes increases cancellous bone volume, but the function of Notch signaling in osteoblast precursors is unknown. To inactivate Notch signaling in immature osteoblastic cells, mice homozygous for conditional Notch1 and Notch2 alleles (Notch1loxP/loxP;Notch2loxP/loxP) were crossed with mice where the osterix (Osx) promoter, regulated by a Tet-Off cassette, governs Cre expression (Osx-Cre). Notch1loxP/loxP;Notch2loxP/loxP control and Osx-Cre+/â;Notch1Î/Î;Notch2Î/Î experimental littermate cohorts were obtained. To prevent the effects of embryonic Osx-Cre expression, doxycycline was administered to pregnant dams, but not to newborns. Recombination of conditional alleles was documented in calvarial DNA extracts from 1Â month old mice. Notch1 and Notch2 inactivation did not affect femoral microarchitecture at 1Â month of age. Cancellous bone volume was higher and structure model index was lower in 3 and 6Â month old Osx-Cre+/â;Notch1Î/Î;Notch2Î/Î mice than in control littermates and the effect was more pronounced in female mice. One month old Osx-Cre+/â;Notch1Î/Î;Notch2Î/Î male mice transiently exhibited an increase in osteoblast number and a modest suppression in bone resorption. Osx-Cre+/â;Notch1Î/Î;Notch2Î/Î female mice displayed a tendency toward increased bone formation at 3Â months of age, although bone remodeling was suppressed in 6Â month old Osx-Cre+/â;Notch1Î/Î;Notch2Î/Î female mice. Notch1 and Notch2 inactivation increased porosity and reduced thickness of cortical bone. These effects were modest and more evident in 3 and 6Â month old female than in male mice of the same age. In conclusion, Notch1 and Notch2 expression in osteoblast precursors regulates cancellous bone volume and microarchitecture.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 62, May 2014, Pages 22-28
Journal: Bone - Volume 62, May 2014, Pages 22-28
نویسندگان
Stefano Zanotti, Ernesto Canalis,