17β Estradiol regulation of connexin 43-based gap junction and mechanosensitivity through classical estrogen receptor pathway in osteocyte-like MLO-Y4 cells

► 17β estradiol (E2) raises Cx43 levels and Cx43-based intercellular communication. ► E2 interaction with oscillating fluid flow affects osteocytic mechanosensitivity. ► Classical estrogen receptors predominantly mediate E2 effects on Cx43.