کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5890961 1153262 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Glucose-dependent insulinotropic polypeptide (GIP) receptor deletion leads to reduced bone strength and quality
ترجمه فارسی عنوان
حذف ضایعه گیرنده پلیپپتید انزوای ناشی از گلوکز منجر به کاهش قدرت استخوانی و کیفیت
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
چکیده انگلیسی


• GIPR deficiency results in lower bone strength.
• GIPR deficient animals present with alterations of cortical bone microarchitecture and fracture resistance.
• Lack of a functional GIPR leads to alterations of intrinsic properties and mineralization of the bone matrix.

Bone is permanently remodeled by a complex network of local, hormonal and neuronal factors that affect osteoclast and osteoblast biology. In this context, a role for gastro-intestinal hormones has been proposed based on evidence that bone resorption dramatically falls after a meal. Glucose-dependent insulinotropic polypeptide (GIP) is one of the candidate hormones as its receptor, glucose-dependent insulinotropic polypeptide receptor (GIPR), is expressed in bone. In the present study we investigated bone strength and quality by three-point bending, quantitative x-ray microradiography, microCT, qBEI and FTIR in a GIPR knockout (GIPR KO) mouse model and compared with control wild-type (WT) animals. Animals with a deletion of the GIPR presented with a significant reduction in ultimate load (-− 11%), stiffness (− 16%), total absorbed (− 28%) and post-yield energies (− 27%) as compared with WT animals. Furthermore, despite no change in bone outer diameter, the bone marrow diameter was significantly increased and as a result cortical thickness was significantly decreased by 20% in GIPR deficient animals. Bone resorption at the endosteal surface was significantly increased whilst bone formation was unchanged in GIPR deficient animals. Deficient animals also presented with a pronounced reduction in the degree of mineralization of bone matrix. Furthermore, the amount of mature cross-links of collagen matrix was significantly reduced in GIPR deficient animals and was associated with lowered intrinsic material properties. Taken together, these data support a positive effect of the GIPR on bone strength and quality.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 56, Issue 2, October 2013, Pages 337–342