Sclerostin expression is induced by BMPs in human Saos-2 osteosarcoma cells but not via direct effects on the sclerostin gene promoter or ECR5 element

► Sclerostin expression enhanced by BMPs and coupled to osteoblast mineralization. ► BMPs, Wnt3a and PTH do not directly activate sclerostin promoter. ► BMP2 increases expression of several sclerostin inhibitors. ► MEF2B increased activity of the promoter and ECR5 element. ► No ECR5 enhancer activity in human osteosarcoma cells.