کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5892257 1153302 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultra-short echo-time MRI detects changes in bone mineralization and water content in OVX rat bone in response to alendronate treatment
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Ultra-short echo-time MRI detects changes in bone mineralization and water content in OVX rat bone in response to alendronate treatment
چکیده انگلیسی

In this work we hypothesize that bisphosphonate treatment following ovariectomy manifests in increased phosphorus and decreased water concentration, both quantifiable nondestructively with ultra-short echo-time (UTE) 31P and 1H-MRI techniques. We evaluated this hypothesis in ovariectomized (OVX) rats undergoing treatment with two regimens of alendronate. Sixty female four-month-old rats were divided into four groups of 15 animals each: ovariectomized (OVX), OVX treatment groups ALN1 and ALN2, receiving 5 µg/kg/day and 25 µg/kg/day of alendronate, and a sham-operated group (NO) serving as control. Treatment, starting 1 week post surgery, lasted for 50 days at which time animals were sacrificed. Whole bones from the left and right femora were extracted from all the animals. 31P and 1H water concentration were measured by UTE MRI at 162 and 400 MHz in the femoral shaft and the results compared with other measures of mineral and matrix properties obtained by 31P solution NMR, CT density, ash weight, and water measured by dehydration. Mechanical parameters (elastic modulus, EM, and ultimate strength, US) were obtained by three-point bending. The following quantities were lower in OVX relative to NO: phosphorus concentration measured by 31P-MRI (−8%; 11.4 ± 0.9 vs. 12.4 ± 0.8%, p < 0.005), 31P-NMR (−4%; 12.8 ± 0.4 vs. 13.3 ± 0.8 %, p < 0.05) and µ-CT density (−2.5%; 1316 ± 34 vs. 1349 ± 32 mg/cm3, p = 0.005). In contrast, water concentration by 1H-MRI was elevated in OVX relative to NO (+ 6%; 15.5 ± 1.7 vs. 14.6 ± 1.4 %, p < 0.05). Alendronate treatment increased phosphorus concentration and decreased water concentration in a dose-dependent manner, the higher dose yielding significant changes relative to values found in OVX animals: 31P-MRI (+ 14%; p < 0.0001), 31P-NMR (+ 9%; p < 0.0001), ash content (+ 1.5%; p < 0.005), µ-CT mineralization density (+ 2.8%; p < 0.05), and 1H-MRI, (−19%, p < 0.0001). The higher dose raised phosphorus concentration above and water concentration below NO levels: 31P-MRI (+ 6%; p < 0.05), 31P-NMR (+ 5%; p = 0.01), ash content (+ 1.5%; p = 0.005), 1H-MRI (−14%; p < 0.0001), and drying water (−10%; p < 0.0005). Finally, the group means of phosphorus concentration were positively correlated with EM and US (R2 ≥ 0.98, p < 0.001 to p < 0.05) even though the pooled data from individual animals were not. The results highlight the implications of estrogen depletion and bisphosphonate treatment on mineral composition and mechanical properties and the potential of solid-state MR imaging to detect these changes in situ in an animal model of rat ovariectomy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 46, Issue 5, May 2010, Pages 1391-1399
نویسندگان
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