کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5899524 1155599 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A flavonoid fraction purified from Rutaceae aurantiae (DaflonR) inhibiting AGE formation, reduces urinary albumin clearance and corrects hypoalbuminemia in normotensive and hypertensive diabetic rats
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
A flavonoid fraction purified from Rutaceae aurantiae (DaflonR) inhibiting AGE formation, reduces urinary albumin clearance and corrects hypoalbuminemia in normotensive and hypertensive diabetic rats
چکیده انگلیسی

AimAdvanced glycation endproducts (AGEs) have been shown to contribute to alteration of glomerular permselectivity to proteins in diabetes. Oxidative stress is required for AGE formation. Therefore we studied the effect of an antioxidant micronized purified flavonoid fraction (MPFF, DaflonR 500 mg), on urinary albumin clearance in diabetic rats.MethodsHyperglycaemia was induced by streptozotocin 55 mg/kg IM at days 0 and 7 in normotensive Wistar rats (NWR, diabetes duration 5 months) or hypertensive Wistar Kyoto rats (SHR, diabetes duration 2 months). MPFF was administered at 300 mg/kg/day, from day −2 until sacrifice.ResultsAfter 5 months of diabetes in NWR, MPFF reduced albumin clearance from 729 ± 92 to 392 ± 60 nl/min/kg, p < 0.01, and restored albuminemia from 20.4 ± 0.9 to 24.0 ± 1 g/l, p < 0.05; albumin fractional clearance was significantly diminished in the flavonoid-treated diabetic rats (0.360 ± 0.037‰ versus 1.335 ± 0.430‰ in the diabetic controls, p < 0.001); MPFF did not significantly modify blood glucose and plasma fructosamine levels. After 2 months of diabetes in SHR, MPFF reduced albumin clearance from 243 ± 121 to 101 ± 47 nl/min/kg, p < 0.05, and restored albuminemia from 21.1 ± 1.6 to 26.7 ± 2.2 g/l (p < 0.05); MPFF also decreased plasma fluorescence characteristic of AGEs (p < 0.02). Besides hesperetin, a main metabolite of MPFF recovered in plasma, inhibited in vitro the formation of the crosslinking AGE pentosidine in collagen incubated with high glucose (p < 0.001).ConclusionOur results confirm the role of glycoxidative stress in diabetic nephropathy. MPFF might be useful as complementary treatment for preventing diabetic microangiopathy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diabetes Research and Clinical Practice - Volume 105, Issue 3, September 2014, Pages 373-381
نویسندگان
, , , , , , , ,