کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905336 | 1569843 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of fibronectin type III domain-containing protein 5 (FNDC5) gene in chickens: Cloning, tissue expression, and regulation of its expression in the muscle by fasting and cold exposure
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کلمات کلیدی
IBMXmESCsFNDC5MVDBATqPCR3-isobutyl-1-methylxanthine - 3-ایزوبوتیل-1-متیلکسانتینUcp1 - UCP1WAT, White adipose tissue - بافت چربی سفید، بافت ادیپوز سفیدbrown adipose tissue - بافت چربی قهوه ایMacrovascular disease - بیماری کلیوی قلبCNS - دستگاه عصبی مرکزیFasting - روزه داریreverse transcription - رونویسی معکوسMouse embryonic stem cells - سلول های بنیادی جنینی موشcentral nervous system - سیستم عصبی مرکزیIrisin - شما ایریناCold exposure - قرار گرفتن در معرض سردEnergy metabolism - متابولیسم انرژیChicken - مُرغ خانگیpolymerase chain reaction - واکنش زنجیره ای پلیمرازquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPCR - واکنش زنجیرهٔ پلیمرازuncoupling protein 1 - پروتئین جدا سازی 1WAT - چی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Irisin, a novel myokine encoded by fibronectin type III domain-containing protein 5 gene (FNDC5), is reported to stimulate brown fat-like development of white fat tissue and thermogenesis in mammals recently. However, information about the structure, tissue expression, and roles of FNDC5/irisin remains unknown in non-mammalian vertebrates including birds. In this study, we first cloned the FNDC5 (cFNDC5) cDNA from chickens. cFNDC5 is predicted to encode a 220-amino acid precursor containing the putative 'irisin peptide' of 112 amino acids, which shows high amino acid sequence identity with irisin of humans (97%), mice (97%), anole lizards (93%) and zebrafish (~Â 80%). Using quantitative real-time PCR, we further examined cFNDC5 mRNA expression in chicken tissues. The results showed that in adult chickens, cFNDC5 is abundantly expressed in the muscle, heart, pituitary, ovary and various brain regions, and moderately expressed in adipose tissue, kidneys, lung, testes and small intestine. Moreover, cFNDC5 is also abundantly expressed in the muscle, brain, hypothalamus and pituitary of developing embryos and post-hatching chicks. Interestingly, we noted that cFNDC5 expression in the muscle of 3-week-old chicks could be induced by fasting and cold exposure, while its expression decreases during differentiation of pre-adipocytes cultured in vitro. Collectively, our data suggest that FNDC5/irisin is more than a 'myokine' and may be related to the development/functions of many tissues (e.g. muscle, brain, fat), as well as metabolic status of chickens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 570, Issue 2, 10 October 2015, Pages 221-229
Journal: Gene - Volume 570, Issue 2, 10 October 2015, Pages 221-229
نویسندگان
Xin Li, Wenqian Fang, Yuanyuan Hu, Yajun Wang, Juan Li,