کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905517 | 1159904 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning of porcine GPIHBP1 gene and its tissue expression pattern and genetic effect on adipose traits
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کلمات کلیدی
SASTOP2BGPIHBP1LPLHWEqRT-PCRORFBFTTBP3′-UTR - 3'-UTR3′-untranslated region - 3'-ناحیه ترجمه نشدهχ2 - x2β-actin - β-اکتینmarker-assisted selection - انتخاب کمک کننده نشانگرTissue expression - بیان بافتMAS - بیشترCloning - تاگسازی یا شبیه سازیHardy–Weinberg equilibrium - تعادل هاردی-وینبرگStatistical Analysis System - سیستم تحلیل آماریIMF - صندوق بین المللی پولback fat thickness - ضخامت چربی پشتActb - عملopen reading frame - قاب خواندن بازLipoprotein lipase - لیپو پروتئین لیپازLipoprotein lipase (LPL) - لیپوپروتئین لیپاز (LPL)quantitative reverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی معکوس و پلیمریزا معکوسTATA box binding protein - پروتئین اتصال دهنده جعبه TATAPolymorphism - پلی مورفیسمSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیIntramuscular fat - چربی داخل عضلانیSNP - چندریختی تک-نوکلئوتیدChi-square - چی مربعPorcine - گوشت خوک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism and is transported by glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 (GPIHBP1) from the interstitial spaces to the capillary lumen. Here, we cloned a cDNA and the genomic locus of the porcine GPIHBP1 gene, and investigated its tissue expression pattern and its genetic effects on adipose traits. Porcine GPIHBP1 exhibits a four-exon/three-intron structure, including a 543Â bp open reading frame that encodes 180 amino acids. The porcine GPIHBP1 protein shows 49%-65% homology and shares the major conserved structural domains of GPIHBP1 proteins in other mammals. Porcine GPIHBP1 mRNA levels were high in the adipose tissue, muscle and lung, and higher mRNA levels were observed in sows compared to boars in adipose tissues of the inner and outer layers of subcutaneous fat, abdominal fat, and suet fat. The mRNA expression pattern of porcine GPIHBP1 and LPL genes was similar in most tissues except for the lung. Thirty six single nucleotide polymorphisms (SNPs) were found in the porcine GPIHBP1 gene. Association analyses showed that the g.-255G>C and g.-626T>G SNPs are associated with intramuscular fat content, and that the g.-1557T>C and g.-1948G>A SNPs are associated with back fat thickness. In conclusion, porcine GPIHBP1 mRNA is abundantly expressed in the adipose tissue, muscle and lung, and gender affects GPIHBP1 mRNA expression levels; furthermore, four GPIHBP1 SNPs are genetic factors affecting adipose traits.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 557, Issue 2, 25 February 2015, Pages 146-153
Journal: Gene - Volume 557, Issue 2, 25 February 2015, Pages 146-153
نویسندگان
Huaming Xu, Xuelian Tao, Yingying Wei, Jianning Chen, Shuhua Xing, Wangmin Cen, Anxiang Wen, Li Zhu, Guoqing Tang, Mingzhou Li, Anan Jiang, Yanzhi Jiang, Xuewei Li,