کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905584 | 1159908 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The investigation of miR-221-3p and PAK1 gene expressions in breast cancer cell lines
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کلمات کلیدی
HER2DMEMqRT-PCRPAK1SERMSRC-1Dulbecco's Modified Eagle's Minimum Essential MediumReal-time PCR - PCR به موقعmicroRNAs - ریز آرانایBreast cancer - سرطان پستانMiRNA - میکروRNA، ریزآرانای، miRNAsteroid receptor coactivator-1 - گیرنده استروئید coactivator-1Human epidermal growth factor receptor 2 - گیرنده عامل فاکتور رشد اپیدرمی انسان 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The investigation of miR-221-3p and PAK1 gene expressions in breast cancer cell lines The investigation of miR-221-3p and PAK1 gene expressions in breast cancer cell lines](/preview/png/5905584.png)
چکیده انگلیسی
The most common malignancy in women is breast cancer. Drug resistance in the treatment of cancer still remains a major clinical concern. Resistance to tamoxifen is seen in half of the recurrences in breast cancer. The anti-estrogen tamoxifen gains agonistic property by transactivating ERα. PAK1-mediated phosphorylation of serine 305 (S305) of ERα leads to resistance to tamoxifen. In our study, PAK1-induced suggestive tamoxifen resistance was designed. According to our hypothesis, phosphorylation of ERα-S305 by PAK1 may be reversed by PAK1 transcriptional inhibition by miR-221-3p due to miR-221-3p targeting the 3ⲠUTR of PAK1. For this purpose, we used Real-time PCR (qRT-PCR) to measure the expression level of miR-221-3p in ER-positive breast cancer cell lines (ZR-75-1, MCF7) and breast epithelial cell line, hTERT-HME1, as control in the laboratory in our department. The increase in the expression of PAK1 depending on miR-221-3p may be related to ZR-75-1 cell line which has invasive characteristic but other two ER + cancer cell lines, MCF7 and HCC1500, have milder cancer severity. miR-221-3p may have a role on regulation of PAK1 expression because miR-221-3p expression level decreases while PAK1 expression level increases in SKBR3 cell line. miR-221-3p and PAK1 expressions in MDA-MB-231 cell line are higher than that of hTERT-HME1 cell line. This may mean that miR-221-3p has no regulatory effect on of PAK1 expression in this cell line. According to these results, miR-221-3p may give crucial information about molecular mechanism of the disease upon PAK1 activity or different mechanisms with respect to histopathology and severity of breast cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 377-381
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 377-381
نویسندگان
Sercan Ergun, Tayeb Sadiq Tayeb, Ahmet Arslan, Ebru Temiz, Kaifee Arman, Muhammad Safdar, Hasan DaÄlı, Murat Korkmaz, Gülper Nacarkahya, Sevil KırkbeÅ, Serdar Oztuzcu,