کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5906118 | 1159953 | 2013 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A R2R3-MYB transcription factor, GmMYB12B2, affects the expression levels of flavonoid biosynthesis genes encoding key enzymes in transgenic Arabidopsis plants
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کلمات کلیدی
qRT-PCRsqRT-PCRTFsTransgenic Arabidopsis thalianaDFRF3HDAPPALCHSMYB transcription factorsCHIIFSF3′H - F3'HExpression pattern - الگوی بیانisoflavone synthase - ایزوفلاون سنتازIsoflavones - ایزوفلاونهاdihydroflavonol 4-reductase - دی هیدروفلوانول 4-ردوکتازdays after pollination - روز بعد از گردهافشانیchalcone synthase - سنتاز چالکونTranscription factors - عوامل رونویسیTranscriptional activation - فعال سازی رونویسیFlavonoid - فلاونوئیدflavanone 3-hydroxylase - فلاونون 3-هیدروکسیلازPhenylalanine ammonia lyase - فنیل آلانین آمونیاک لیائازquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کاراchalcone isomerase - کلومون ایزومراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Isoflavones play diverse roles in plant-microbe interactions and are potentially important for human nutrition and health. To study the regulation of isoflavonoid synthesis in soybean, the R2R3-MYB transcription factor GmMYB12B2 was isolated and characterized. Yeast expression experiments demonstrated that GmMYB12B2 showed transcriptional activity. GmMYB12B2 was localized in the nucleus when it was transiently expressed in onion epidermal cells. Real-time quantitative PCR analysis revealed that GmMYB12B2 transcription was increased in roots and mature seeds compared with other organs. The gene expression level in immature embryos was consistent with the accumulation of isoflavones. CHS8 is a key enzyme in plant flavonoid biosynthesis. Transient expression experiments in soybean calli demonstrated that CHS8 was regulated by GmMYB12B2 and produced more fluorescence. The expression levels of some key enzymes in flavonoid biosynthesis were examined in transgenic Arabidopsis lines. The results showed that the expression levels of PAL1, CHS and FLS in transgenic plants were significantly higher than those in wild type plants. However, the expression level of DFR was lower, and the expression levels of CHI, F3H and F3â²H were the same in all lines. GmMYB12B2 expression caused a constitutive increase in the accumulation of flavonoids in transgenic Arabidopsis lines compared with wild type plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 532, Issue 1, 10 December 2013, Pages 72-79
Journal: Gene - Volume 532, Issue 1, 10 December 2013, Pages 72-79
نویسندگان
Xiao-Wei Li, Jing-Wen Li, Ying Zhai, Yan Zhao, Xu Zhao, Hai-Jun Zhang, Lian-Tai Su, Ying Wang, Qing-Yu Wang,