کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5906521 | 1159973 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapamycin preserves the follicle pool reserve and prolongs the ovarian lifespan of female rats via modulating mTOR activation and sirtuin expression
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کلمات کلیدی
mTORS6 kinase 1RPS6POFmTORC1SirtuinsS6K1p70S6KSIRTTSCFRAPcorpora lutea - اجسام زردsilent information regulator - تنظیم کننده اطلاعات خاموشRapamycin - راپامایسینphosphatase and tensin homolog deleted on chromosome 10 - فسفاتاز و هومولوگ تنسین حذف شده در کروموزوم 10follicle - فولیکولcalorie restriction - محدودیت کالریpremature ovarian failure - نارسایی زودرس تخمدانthe mammalian target of rapamycin - هدف پستانداران رپامایسینRibosomal protein S6 - پروتئین Ribosomal S6ribosomal protein S6 kinase - پروتئین ریبوزومی S6 کینازPten - ژن PTENTuberous sclerosis complex - کمپلکس توبروس اسکلروزیس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Rapamycin preserves the follicle pool reserve and prolongs the ovarian lifespan of female rats via modulating mTOR activation and sirtuin expression Rapamycin preserves the follicle pool reserve and prolongs the ovarian lifespan of female rats via modulating mTOR activation and sirtuin expression](/preview/png/5906521.png)
چکیده انگلیسی
To maintain the normal length of female reproductive life, the majority of primordial follicles must be maintained in a quiescent state for later use. In this study, we aimed to study the effects of rapamycin on primordial follicle development and investigate the role of mTOR and sirtuin signaling. Rats were treated every other day with an intraperitoneal injection of rapamycin (5Â mg/kg) or vehicle. After 10Â weeks of treatment, ovaries were harvested for hematoxylin and eosin (HE) staining, and analysis by immunohistochemistry and Western blotting. HE staining showed that the number and percentage of primordial follicles in the rapamycin-treated group were twice the control group (PÂ <Â 0.001). Immunohistochemical analysis showed that mTOR and phosphorylated-p70S6K were extensively expressed in surviving follicles with strong staining observed in the cytoplasm of the oocyte. Western blotting showed decreased expression of phosphorylated mTOR and phosphorylated p70S6K in the rapamycin-treated group, and increased the expression of both SIRT1 and SIRT6 compared to the control group (PÂ <Â 0.05). Taken together, these results suggest that rapamycin may inhibit the transition from primordial to developing follicles and preserve the follicle pool reserve, thus extending the ovarian lifespan of female rats via the modulation of mTOR and sirtuin signalings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 523, Issue 1, 1 July 2013, Pages 82-87
Journal: Gene - Volume 523, Issue 1, 1 July 2013, Pages 82-87
نویسندگان
Xing-mei Zhang, Li Li, Jin-jie Xu, Na Wang, Wei-juan Liu, Xuan-hao Lin, Yu-cai Fu, Li-li Luo,