Replication timing in a single human chromosome 11 transferred into the Chinese hamster ovary (CHO) cell line

► CHO cells were engineered to carry a single human chromosome 11. ► Replication timing program at an R/G boundary was maintained in the CHO(h11) cells. ► Replication timing at the centromere was also similar to the original fibroblasts. ► The 11q telomere showed abnormally early replication in CHO(h11) cells. ► The telomere region may have a specific mechanism for regulating replication timing.