کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5907180 1160002 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and characterization of a muskmelon cDNA encoding Lycopene Beta-cyclase
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Isolation and characterization of a muskmelon cDNA encoding Lycopene Beta-cyclase
چکیده انگلیسی

Lycopene Beta-cyclase (LCY-B) is thought to play a critical role in Beta‐carotene synthesis in fruit. A full-length cDNA clone encoding Lycopene Beta-cyclase was isolated from muskmelon (Cucumis melo L.) by RT-PCR and RACE. The clone, designated CmLcyb1, contains 1871 nucleotides, with an open reading frame of 1512 nucleotides. The deduced 504-amino-acid sequence showed high identities with other plant Lycopene Beta-cyclases. Real time quantitative RT-PCR analysis indicated that CmLcyb1 was expressed in all tissues and organs of muskmelon inbred M01-3 with white mesocarp and, 'Homoka', an orange mesocarp cultivar. The expression levels of CmLcyb1 in roots, stems, leaves and flowers in the two genotypes differed little. The expression level was highest in mature fruit of 'Homoka' and was much higher than that in mature fruit of M01-3. Moreover, the mRNA level of CmLcyb1 was very low in fruits before fruit-size fixation and increased dramatically in the size-fixed fruits of these two genotypes. The mRNA levels of CmLcyb1 during fruit development of 'Homoka' were all higher than those of M01-3. Interestingly, Beta‐carotene content showed almost the same change trend as mRNA levels during fruit development in these two genotypes, suggesting that Beta‐carotene accumulation may be linked to the CmLcyb1 transcript level in muskmelon fruit.

► A full-length cDNA encoding Lycopene Beta-cyclase was isolated from melon. ► The expression levels of CmLcyb1 in tissues and organs of melon were analyzed. ► Regulation of CmLcyb1 mRNA level and β‐carotene content in melon fruit were analyzed. ► β‐carotene accumulation may be linked to CmLcyb1 transcript level in melon fruit.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 503, Issue 1, 15 July 2012, Pages 147-151
نویسندگان
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