کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5907530 | 1160025 | 2012 | 6 صفحه PDF | دانلود رایگان |

The epididymis plays a crucial role in regulating the development of sperm motility and fertilizing capacity. Small non-coding RNAs (sncRNAs), especially microRNAs (miRNAs), can participate in the regulation of various physiological pathways. However, their abundance and whether they are involved in the regulation of gene expression in the human epididymis are unknown. By adopting the Solexa deep sequencing approach, we systematically investigated the sncRNAs in the adult human epididymis. A total of 4903 unique sequences representing 527 known miRNA were discovered. Eighteen novel miRNA genes encoding 23 mature miRNAs were also identified and the expression of some of them was confirmed by qRT-PCR. The presence of Piwi-interacting RNAs (piRNAs) in the library also adds to the diversity of the sncRNA population in the human epididymis. This research will contribute to a preliminary database for their functional study in male reproductive system.
⺠The sncRNA species were systematically investigated in the human epididymis. ⺠527 known and 18 novel miRNA candidates were identified. ⺠Numerous piRNAs were identified in the human epididymis for the first time. ⺠This work lays the foundation for clarifying the sncRNA functions in epididymis.
Journal: Gene - Volume 497, Issue 2, 15 April 2012, Pages 330-335