کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5908 445 2014 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A general strategy to achieve ultra-high gene transfection efficiency using lipid-nanoparticle composites
ترجمه فارسی عنوان
یک استراتژی کلی برای دستیابی به کارایی ترانسفکشن ژن فوق فوقانی با استفاده از کامپوزیتهای لیپید-نانوذرات
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

Gene therapy provides a new hope for previously “incurable” diseases. Low gene transfection efficiency, however, is the bottle-neck to the success of gene therapy. It is very challenging to develop non-viral nanocarriers to achieve ultra-high gene transfection efficiencies. Herein, we report a novel design of “tight binding-but-detachable” lipid-nanoparticle composite to achieve ultrahigh gene transfection efficiencies of 60∼82%, approaching the best value (∼90%) obtained using viral vectors. We show that Fe@CNPs nanoparticles coated with LP-2000 lipid molecules can be used as gene carriers to achieve ultra-high (60–80%) gene transfection efficiencies in HeLa, U-87MG, and TRAMP-C1 cells. In contrast, Fe@CNPs having surface-covalently bound N,N,N-trimethyl-N-2-methacryloxyethyl ammonium chloride (TMAEA) oligomers can only achieve low (23–28%) gene transfection efficiencies. Similarly ultrahigh gene transfection/expression was also observed in zebrafish model using lipid-coated Fe@CNPs as gene carriers. Evidences for tight binding and detachability of DNA from lipid-nanoparticle nanocarriers will be presented.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 35, Issue 28, September 2014, Pages 8261–8272
نویسندگان
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